The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs.
نویسندگان
چکیده
The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that this method has high specificity and sensitivity and can be applied in analytical laboratories in diagnosis of canine babesiosis.
منابع مشابه
Development and evaluation of a loop-mediated isothermal amplification assay for detection of Ehrlichia canis DNA in naturally infected dogs using the p30 gene.
Canine monocytic ehrlichiosis (CME) is a common tick-borne disease caused by the rickettsial bacterium Ehrlichia canis (Rickettsiales: Anaplasmataceae). In view of the different stages and variable clinical signs of CME, which can overlap with those of other infections, a conclusive diagnosis can more readily be obtained by combining clinical and hematological evaluations with molecular diagnos...
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The aim of the present study was to evaluate usefulness of PCR assay for the diagnosis of atypical cases of the Babesia canis infections. Primers designed based on the 18 S RNA gene sequence of B. canis. The location of the primers was targeted to evolutionary conserved region. Blood samples from dogs suspected for babesiosis were analysed microscopically and then by PCR. The PCR assay showed h...
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ورودعنوان ژورنال:
- Polish journal of veterinary sciences
دوره 16 1 شماره
صفحات -
تاریخ انتشار 2013